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@#AIM:To observe the curative effect of take oral and cold-wet compress on eyes with Tiaoti Tuomin Decoction and the changes of immunologic function in patients with allergic conjunctivitis. METHODS:Totally 160 patients 320 eyes with allergic conjunctivitis were randomly divided into observation group and control group. The 80 patients(160 eyes)in the observation group were treated with Tiaoti Tuomin Decoction take oral and cold-wet compress on eyes. The 80 patients(160 eyes)in the control group were treated with 0.05% Azelastine Hydrochloride Eye Drops. After 14d of continuous treatment, the symptom and sign scores, quality of life questionnaire scores of allergic conjunctivitis, and the levels of serum immunoglobulin IgG, IgA, IgE of the two groups were compared and analyzed.RESULTS:After treatment, the scores of symptom and sign, quality of life questionnaire of allergic conjunctivitis in both groups were better than those before treatment(<i>P</i><0.05), and the improvement degree of which in the observation group was better than that in the control group(<i>P</i><0.05). After treatment, the levels of serum immunoglobulin IgG, IgA in the two groups were not significant improvement than before treatment(<i>P</i> >0.05), the level of serum immunoglobulin IgE in the observation group were significantly improved than before treatment(<i>P</i><0.05). CONCLUSION: Treatment of take oral and cold-wet compress on eyes with Tiaoti Tuomin Decoction can improve clinical symptoms of allergic conjunctivitis patients, help relieve allergic reaction and improve the quality of life.
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Post-traumatic stress disorder (PTSD) is a reactive mental disorder that occurs after an individual was exposed to a traumatic event,and the core of the treatment is the extinction of conditioned fear memory caused by stress.Fear memory is an incentive mechanism based on external stimuli that occupy a central position in the defense system.Traditional memory concept convinced that the original memory traces were in an unstable state when the memory was activated.This process is called memory reconsolidation.The research has proved the existence of the fear memory reconsolidation,but the specific mechanism of reconsolidation has not been clarified.The animal studies show that many brain sites and molecular mechanisms are involved in the process of fear memory reconsolidation.Understanding the underlying mechanisms of fear memory reconsolidation is conducive to the treatment of specific phobias and PTSD.This review summarized the brain structure and molecular mechanism of conditioned fear memory reconsolidation,providing a new direction for the in-depth study of conditioned fear memory reconsolidation and PTSD.
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Post-traumatic stress disorder (PTSD) is a reactive mental disorder that occurs after an individual was exposed to a traumatic event,and the core of the treatment is the extinction of conditioned fear memory caused by stress.Fear memory is an incentive mechanism based on external stimuli that occupy a central position in the defense system.Traditional memory concept convinced that the original memory traces were in an unstable state when the memory was activated.This process is called memory reconsolidation.The research has proved the existence of the fear memory reconsolidation,but the specific mechanism of reconsolidation has not been clarified.The animal studies show that many brain sites and molecular mechanisms are involved in the process of fear memory reconsolidation.Understanding the underlying mechanisms of fear memory reconsolidation is conducive to the treatment of specific phobias and PTSD.This review summarized the brain structure and molecular mechanism of conditioned fear memory reconsolidation,providing a new direction for the in-depth study of conditioned fear memory reconsolidation and PTSD.
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AlM: To observe retinal hemodynamic influence of compound xueshuantong capsule on nonproliferative diabetic retinopathy ( NPDR) after laser photocoagulation.METHODS:A total of 41 patients (72 eyes) with NPDR after laser photocoagulation were enrolled in this study. They were all given compound xueshuantong capsule, and used color Doppler flow imaging for detection of retinal hemodynamics. RESULTS: After treatment, patients with retinal blood perfusion significantly improved; central retinal arterial peak systolic velocity ( PSV ) , end - diastolic velocity (EDV) and medial velocity (Vm) were increased, while the resistance index ( Rl) decreased. The difference have statistical significance (P CONCLUSlON: Compound xueshuantong capsule can improve retinal blood perfusion for nonproliferative diabetic retinopathy after laser photocoagulation, which is related to improvement of visual prognosis.
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Objective To investigate the effect of the standing position on the amplitudes of QRS waves of 12-lead electrocardiogram (ECG). Methods The ECG was recorded in the supine and standing positions in 60 hospitalized patients or out-patients, and the amplitudes of Q, R, and S waves of all 12 ECG leads were measured and compared between the two positions. Results In inferior leads, the amplitudes of Q waves and R waves in leads II, III and aVF recorded in standing position were significantly deepened or increased, and S waves in lead III recorded in standing position were significantly shallowed compared with those in the supine position(P<0. 05). In lateral leads, the amplitudes of Q waves in leads I and V5-6, S waves in leads I and aVL recorded in the standing positions were significantly shallowed compared with those in the supine positions; R waves in leads I and aVL recorded in the standing positions were decreased compared with those in the supine position (P<0. 05). In thoracic leads, the amplitudes of R waves in leads V1-4 recorded in the standing position were significantly decreased compared with those in the supine position; the S waves in leads V1-3 waves recorded in the standing position were significantly shallowed compared with that in the supine position (P-< 0. 05); and the R waves of lead aVR recorded in the standing position was significantly increased compared with that in the supine position (P<0. 05). Conclusion The amplitudes of QRS waves of ECG are different when recorded in the standing and supine positions.
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<p><b>BACKGROUND</b>The genotype of epidermal growth factor receptor (EGFR) is associated with tyrosine kinase inhibitor and effectiveness of therapy, but its role in cytotoxic chemotherapy is still unknown. Previous studies indicated that certain EGFR mutations were associated with response and progression free survival following platinum based chemotherapy. Our recent studies have identified that EGFR genotypes in the tumour tissues were not associated with response to the first-line chemotherapy in Chinese patients with advanced non-small cell lung cancer (NSCLC). In this study, we investigated associations of EGFR genotypes from plasma of patients with advanced NSCLC and response to first-line chemotherapy and prognosis.</p><p><b>METHODS</b>We enrolled 145 advanced NSCLC patients who had received first-line chemotherapy in our department. We examined plasma EGFR genotypes for these patients and associations of EGFR mutations with response to chemotherapy and clinical outcomes.</p><p><b>RESULTS</b>There were 54 patients with known EGFR mutations and 91 cases of wild types. No significant difference was detected in the response rate to first-line chemotherapy between mutation carriers and wild-type patients (37.0% vs. 31.9%). The median survival time and 1-, 2-year survival rates were higher in mutation carriers than wild-types (24 months vs. 18 months, 85.7% vs. 65.7% and 43.7% vs. 25.9%, P = 0.047). Clinical stage (IV vs. IIIb), response to the first-line chemotherapy (partial vs. no) and EGFR genotype were independent prognostic factors.</p><p><b>CONCLUSION</b>Plasma EGFR mutations in the Chinese patients with advanced NSCLC is not a predictor for the response to first-line chemotherapy, but an independent prognostic factor indicating longer survival.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Antineoplastic Agents , Therapeutic Uses , Carcinoma, Non-Small-Cell Lung , Drug Therapy , Genetics , Genotype , Plasmids , Genetics , ErbB Receptors , Genetics , Survival RateABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effect of therapeutic ultrasound-induced microbubble's cavitation on plasmid gene transduction in rat pulmonary endothelial cells in relation to the changes of membrane fluidity and cytoskeleton structure.</p><p><b>METHODS</b>Rat endothelial cells cultured in vitro were transfected with EGFP plasmid in the presence of protein microbubbles. During the transfection process, the cells were exposed to continuous 2 MHz ultrasonic irradiation for 30, 60, 90, 120 and 180 s (groups A, B, C, D and E, respectively) with the constant mechanical index (MI) of 1.0, or for 60 s with different mechanical index (MI) of 0.5, 0.75, 1.0, 1.5, and 1.8 (groups B1, B2, B3, B4 and B5, respectively). The changes of endothelial cytoskeletal structure and membrane fluidity were evaluated by immunofluorescence staining after the exposure.</p><p><b>RESULTS</b>EGFP gene transduction increase obviously with prolonged echo irradiation and increased MI. The intensity of immunofluorescence staining, which represented endothelial membrane fluidity, was 0.173±0.013, 0.250±0.037, 0.364±0.022, 0.381±0.019, and 0.395±0.009 in groups A-E, as compared with 0.171±0.017, 0.255±0.026, 0.378±0.007, 0.382±0.009 and 0.397±0.008 in groups B1-B5, respectively. The recovery intensity of the immunofluorescence staining representing the changes in microtubulin of the cytoskeleton structure was 159.15±4.79, 188.23±6.20, 205.80±4.48, 208.99±8.34, and 213.70±5.09 in groups A-E, and was 176.84±3.10, 187.57±14.52, 206.41±11.66, 220.12±13.39 and 221.16±12.78 in groups B1-B5, respectively. The endothelial membrane fluidity and microtubule fluorescence recovery intensity increased remarkably compared with the baseline (P<0.01) within the MI range of 0.50-1.0 and the exposure time of 30-90 s, but underwent no further changes in response to prolonged exposure time (180 s) at the MI of 1.5 (P>0.05). No changes in microfilament fluorescence intensity were observed after exposure to different MI or irradiation time.</p><p><b>CONCLUSION</b>Therapeutic ultrasound-mediated albumin microbubble cavitation allows enhances plasmid gene transduction without causing cytoskeleton damages. Increased endothelial membrane fluidity and changes in cytoskeleton structure, especially microtubulin, partially contribute to this enhancement.</p>
Subject(s)
Animals , Rats , Cells, Cultured , Cytoskeleton , Endothelial Cells , Lung , Cell Biology , Membrane Fluidity , Microbubbles , Plasmids , Rats, Sprague-Dawley , Sonication , TransfectionABSTRACT
<p><b>OBJECTIVE</b>To observe the clinical therapeutic effect of combination of Source Point and Back-Shu Point on perimenopausal syndrome, and provide a new thought of therapy for perimenopausal syndrome.</p><p><b>METHODS</b>Eighty cases of perimenopausal syndrome were randomly divided into a combination of Source Point and Back-Shu Point group and a routine acupuncture group, 40 cases in each group. The combination of Source Point and Back-Shu Point group was treated with acupuncture at Taixi (KI 3), Taichong (LR 3), Taibai (SP 3), Sanyinjiao (SP 6), Shenshu (BL 23), Ganshu (BL 18), Pishu (BL 20), Guanyuan (CV 4); and the routine acupuncture group was treated with acupuncture at Baihui (GV 20), Guanyuan (CV 4), Shenshu (BL 23), Taixi (KI 3), San-yinjiao (SP 6). The Kupperman score and the changes of serum estradiol (E2), follicle stimulating hormone (FSH) and luteinizing hormone (LH) were observed before and after treatment in the two groups.</p><p><b>RESULTS</b>The total effective rate of 92.5% in the combination of Source Point and Back-Shu Point group was superior to 80.0% in the routine acupuncture group, with a significant difference (P < 0. 05); the Kupperman score of 24. 85 +/- 8.35 in the combination of Source Point and Back-Shu Point group was significantly decreased as compared with 35.38 +/- 9.83 in the routine acupuncture group (P < 0.05) after treatment; the contents of E2, FSH and LH in the combination of Source Point and Back-Shu Point group were significantly improved after treatment, and with a significant difference compared to the routine acupuncture group (P < 0.01, P < 0.05).</p><p><b>CONCLUSION</b>Combination of Source Point and Back-Shu Point has a benign regulation function for E2, FSH and LH, and can significantly improve the Kupperman score, is superior to routine acupuncture for perimenopausal syndrome.</p>
Subject(s)
Female , Humans , Middle Aged , Acupuncture , Methods , Acupuncture Points , Acupuncture Therapy , Methods , Estradiol , Blood , Follicle Stimulating Hormone , Blood , Luteinizing Hormone , Blood , Perimenopause , Blood , Syndrome , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To investigate the surface markers of adipose-derived stromal cells (ASCs) with high potential of adipogenic differentiation for increasing efficiency of adipose tissue engineering with selected seeds cells.</p><p><b>METHODS</b>ASCs were harvested from human adipose tissue by collagenase digestion. After proliferation and adipogenic induction of ASCs, the mature induced adipocytes floating in the induction medium were collected. Ceiling culture was used to culture adipocytes and then dedifferentiated adipocytes was obtained at the ceiling. The reproductive activity, adipogenic differentiation potency and expression of surface markers were compared between the dedifferentiated adipocytes and ASCs.</p><p><b>RESULTS</b>The reproductive activity between the dedifferentiation adipocytes and ASCs were similar. The potential of adipogenic differentiation of the dedifferentiated adipocytes was stronger than that of the ASCs. The expression of cell surface markers of both cells were almost the same. But the CD54 positive expression in dedifferentiated adipocytes was higher than that in ASCs.</p><p><b>CONCLUSION</b>The CD54 expression maybe closely associated with high potential of adipogenic differentiation of dedifferentiated adipocytes. CU54 maybe the specific identification of cell surface marker of ASCs with high adipogenic differentiation.</p>
Subject(s)
Humans , Adipocytes , Cell Biology , Adipose Tissue , Cell Biology , Antigens, Surface , Cell Culture Techniques , Cell Dedifferentiation , Cell Differentiation , Cells, Cultured , Stromal Cells , Cell Biology , Tissue Engineering , MethodsABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of therapeutic ultrasound on enhancing albumin microbubble-mediated gene delivery and evaluate the cytoskeletal damages in endothelial cells (ECs).</p><p><b>METHODS</b>The ECs cultured in 6-well plates were transfected with the plasmid eGFP in the presence or absence of the microbubbles at different concentrations, followed by ultrasound exposure for 30 to 180 s at 2 MHz, with the mechanical index (MI) of 0.5-1.8. The gene transfection efficiency was evaluated by observing the green fluorescence of the cells, and the cytoskeletal damage was assessed using immunofluorescence staining 24 to 48 h after ultrasound exposure.</p><p><b>RESULTS</b>Within the MI range of ultrasound exposure between 0.50 and 1.00, the gene transfection efficiency was positively correlated to MI (P<0.001), and further increment of the MI failed to further increase the transfection efficiency. The duration of ultrasound exposure, within the range of 30-90 s, was also positively correlated to the gene transfection efficiency and microtubule fluorescence intensity(P<0.001), and prolonged exposure did not further enhance the effects. Variation of the MI and ultrasound exposure time within the above ranges did not cause significant changes in the florescence intensity of the microfilaments (P>0.05).</p><p><b>CONCLUSION</b>Albumin microbubbles in the presence of ultrasound exposure can substantially enhance the gene transfection efficiency due to therapeutic ultrasound-mediated microbubble destruction without causing obvious damage of the cytoskeletons, and allows safe and efficient nonviral gene delivery in gene therapy.</p>
Subject(s)
Animals , Rats , Cells, Cultured , Contrast Media , Pharmacology , Cytoskeleton , Metabolism , Dose-Response Relationship, Drug , Endothelial Cells , Cell Biology , Metabolism , Fluorescent Antibody Technique , Green Fluorescent Proteins , Genetics , Metabolism , Microbubbles , Microscopy, Fluorescence , Rats, Sprague-Dawley , Time Factors , Transfection , Methods , UltrasonicsABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effects of estrogen on the mitochondria in human umbilical vascular endothelial cells (HUVECs).</p><p><b>METHODS</b>HUVECs were exposed to H2O2 at 250 micromol/L for 4 h with or without pretreatment with 17-estradiol (E2) and ICI182780. Complex IV activity of the cells was measured with chromometry, and 2, 7-dichlorofluorescein diacetate (DCFH-DA) was used to determine intracellular reactive oxygen species (ROS). Intracellular adenosine triphosphate (ATP) level was quantified with a luciferin- and luciferase-based assay.</p><p><b>RESULTS</b>Compared to the blank control group, H2O2 caused a decrease in complex IV activity, intracellular ATP level, and the cell viability, but elevated intracellular ROS. E2 pretreatment of cells significantly attenuated these effects of H2O2 exposure. ICI182780 administered prior to E2 pretreatment antagonized the protective effects of E2 against H2O2 exposure.</p><p><b>CONCLUSION</b>E2 offers mitochondrial protective effects on HUVECs, which is mediated by the estrogen receptors.</p>